Automated Liquid-Liquid Extraction of 25-Hydroxy Vitamin D from Serum and Preparation for LC/MS Analysis Using the Hamilton Robotics Microlab® STARlet Workstation

There are several methods available to measure Vitamin D from blood serum samples, of which LC/MS is the most widely accepted in clinical labs. This application note describes the steps involved for automated extraction of 25-Hydroxy Vitamin D from blood serum and sample preparation for LC/MS analysis. An automated approach to this application eliminates tedious manual processes, resulting in an estimated 600% increase in processing and analysis efficiency.