Automated qPCR master mix preparation with the D-ONE single channel pipetting module and the ASSIST PLUS pipetting robot

Automating qPCR master mix preparation is often considered tedious due to the need to constantly change program set-up based on varying master mix volumes and sample numbers. This application note demonstrates how the D-ONE single channel pipetting module for the ASSIST PLUS pipetting robot is designed to simplify the programming of automated qPCR master mix preparation protocols down to a single step. Here, the D-ONE and ASSIST PLUS is used to verify a SYBR^® Green qPCR protocol prepared directly in a divided reservoir.