Automated single-cell cloning of CHO-S cells using animal-component free conditions in nanolitre volumes

Cell line development (CLD) aims to engineer cell lines that produce recombinant proteins and antibodies with high titres, stringent quality and highest specificity. A critical yet challenging and rate limiting step as part of CLD is the generation of clonal cultures verifiably originating from a single cell. Single-cell cloning remains a tedious, lengthy and inefficient process. Chinese Hamster Ovary (CHO) cells are the main cell type utilized for CLD projects. Explore iotaSciences' assessment of the Cloning Platform for the automated cloning of single CHO cells under animal-component free (ACF) culture media conditions using only nanolitre volumes, while providing whole chamber images of single cells to verify monoclonality of derived cultures.