Automated viral plaque assay workflow using the Cytation Cell Imaging Multimode Reader

Plaque assays remain the standard method for determining viral titers for lysogenic viruses. The assay relies on determining the number of plaque-forming units (PFU) created in a monolayer of virus-infected cells. In this application note, Agilent presents an assay that provides an accurate and efficient method for determining plaque counts and calculating viral titers in a range of microplate densities, including 6-, 24-, 48-, and 96-well formats.