Automating a Linear Density Gradient for Purification of a Protein:Ligand Complex

Proteins have variety of cellular functions, structures and mechanisms of action, routinely binding other biomolecules, or ligands, in order to complete a task. This application note describes the use of the Biomek 4000 Workstation in a simplified method for gradient preparations. To eliminate user variability, Beckman Coulter’s Biomek 4000 Workstation provides consistent and reproducible results in layering discontinuous density gradients.