Automating complex manual sample preparation for mass spec-based proteomics using the Opentrons OT-2 platform

As quantitative proteomics experiments increase in scale and complexity, greater consistency and precision are required, which is best delivered by automation. Dr. Joao Paulo and his laboratory at the Harvard Medical School Department of Cell Biology employ mass spectrometry-based proteomics (MSP) analysis to better understand the effects of molecular mechanisms and cell signaling pathways on the regulation of the proteome of various model systems. He sought to replace labor-intensive manual sample preparation with automation, and his work serves as a demonstrated case study for successful automation of sample preparation for mass spectrometry-based proteomics using the Opentrons OT-2.