Automation and Image-Based Analysis of a Hanging Drop MicroHole Plate Model to Create and Differentiate 3D Mesenchymal Stem Cell Spheroids for Downstream Tissue Formation

Human mesenchymal stem cells (hMSCs) have recently emerged as a leading candidate in cellular therapies due to their unique properties. This application note demonstrates the ability to automate the steps necessary to place hMSCs into microplate inserts, track spheroid formation, and monitor spheroids during differentiation into chondrocyte lineages.