Automation of Homogeneous Proximity Assays for Detection of Erk1/2 or Smad3 Phosphorylation

Cell signaling is generally initiated by cell surface receptor ligand binding events resulting in activation of serine/threonine receptor kinases. This study investigates the performance of two homogeneous high-throughput screening assays capable of screening both modulators of receptor activation as well as intracellularly acting agents, such as inhibitors of upstream events. The assays were coupled to BioTek’s Cytation™ 5 and MultiFlo™ FX Dispenser for increased throughput.