Automation of Non-Alcoholic Fatty Liver Disease Model Assay

Non-alcoholic fatty liver disease is a common malady whose major feature is fat accumulation in the liver, which can lead to liver damage and cirrhosis. One phenotypic marker for this disease is the presence of intracellular neutral lipid droplets. An in vitro model for this phenotype uses the exposure of cells to free fatty acids to elicit the response. Here we describe the automation of the assay workflow using a BioSpa™ 8 Automated Incubator linked to a Cytation™ 5 Cell Imaging Multi-Mode Reader and a MultiFlo™ FX Microplate Dispenser.