Automation of the Agencourt AMPure XP PCR purification system
6 Sept 2023In this application note, Gilson introduces an automated method for the Agencourt® AMPure® XP PCR purification system using PIPETMAX®. This method simplifies and enhances the reliability and flexibility of a typically laborious and technically challenging process. Genomic DNA from Escherichia coli was prepared for whole genome sequencing using the Illumina® Nextera® XT DNA Library Preparation Kit. The Agencourt AMPure XP PCR purification system is employed to clean up the library post-amplification and before normalization. A custom magnetic bead separator rack allows efficient purification of up to 96 samples in a single run. The automated protocol is adjustable, accommodating various factors like sample quantity, volume, bead volume, wash steps, and incubation times to suit specific application needs. This application enables seamless integration of PIPETMAX into diverse workflows.
