Automation of the Illumina® TruSeq® DNA PCR-Free Sample Preparation Kit on the Biomek FXP Automated Liquid Handler

PCR amplification in library preparation can lead to nucleic acid composition bias and has the risk of introducing PCR artifacts. This application note describes the automation of a method for PCR-free preparation of libraries using the Illumina TruSeq DNA PCR-Free Sample Preparation Kit on the Beckman Coulter Biomek FXP Dual-Arm System, Multichannel Pipettor and Span-8 Pipettor automated liquid handler (Biomek FXP). It demonstrates that the method allows the user to create up to 96 individually barcoded, sequence-ready, amplification-free DNA libraries per method run.