Batch Immobilization of a Biotinylated Ligand onto Streptavidin Biosensors

This technical note outlines a general batch mode procedure for immobilizing a biotinylated ligand onto Streptavidin biosensors in the biosensor tray assembly on the benchtop. The goal is to develop a procedure that creates a binding surface having a maximum and reproducible response. The protocol outlines determining the optimal concentration and time for immobilization on-line and then the transfer of these parameters to off-line immobilization.