Best practices for optimizing cell line development processes

The steps to create a stable, manufacturing cell line include host cell line selection, engineering of the expression vector, transfection, cloning and cell expansion, as well as various screening steps to select the clone with the highest cell viability, growth, expression level, stability and product quality. In this eBook, Dr. Jon Dempsey discusses his thoughts regarding best practices for optimizing cell line development (CLD) processes.

Dr. Jon Dempsey is the CEO and Founding Partner of Pathway Biopharma. Dempsey has spent nearly 30 years working in the Biopharmaceutical industry, bringing expertise in biologics chemistry, manufacture and control (CMC) including cell line development, cell therapy and process development, analytics and protein chemistry, CDMO selection and manufacturing. During this time, Dempsey has been involved in the development of several commercial biotherapeutics. In this eBook, Dempsey shares advice for those researchers/ scientists starting out and for those currently in CLD, including tips on the best strategies for process improvement.