Best Workflow Practices for ChIP-seq Analysis with Small Samples

Combined chromatin immunoprecipitation and next-generation sequencing (ChIP-seq) has become the gold standard to investigate genome-wide epigenetic profiles, however this method typically requires a large number of cells. This application note describes a method for carrying out ChIP-seq with a small numbers of cells, using the True MicroChIP Kit and the MicroPlex Library Preparation™ Kit from Diagenode and Advanced Analytical Technologies, Inc. (AATI). This enables reliable analysis of rare or difficult-to-obtain cell types.