Beyond the rainbow: How to multiplex beyond the spectral limitations in multicolor confocal microscopy

Good spectral separation of fluorophores in multicolor confocal microscopy is fundamental to achieving high quality results and being able to include more fluorescent probes in your experiment.

In this review article Leica Microsystems explore:

• Common challenges in spectral separation
• Several strategies you can take to improve the separation of fluorophores
• An alternative technology - Fluorescence Lifetime imaging to separate fluorophores with overlapping spectra