Bile Acid Profiling Using UltraPerformance Convergence Chromatography (UPC²) Coupled to ESI-MS/MS

This application note demonstrates that the use of UltraPerformance Convergence (UPC²) in conjunction with ESI-MS/MS enables the simultaneous profiling of 25 bile acids including conjugated forms in biological fluids. This application demonstrates that this novel separation method is highly suitable for determining the effects of bile acid levels in triglyceride, cholesterol, and glucose metabolism and has a potential to speed up the development of novel drug targets for metabolic diseases.