Biomarker Quantitation in Human Plasma by Multiple Reaction Monitoring (MRM)

In this application note an effective tool for the absolute quantitation of targeted protein by mass spectrometry based multiple reaction monitoring (MRM) is described. In this investigation, nine signature peptides were used for quantitation of the original seven proteins fortified into female human plasma. The accuracy, precision, sensitivity, and linearity of targeted protein quantitation by MRM were evaluated. Two human proteins and five from other species previously detected at low levels were confidently quantified. The results explain why MRM is quickly becoming the standard for protein biomarker verification.