Biosensor roGFP2 Utilized to Assess Cellular Redox Processes

roGFP fusion proteins proved to be a very useful tool to monitor cellular redox changes. Recently, an assay has been developed where roGFP2 was fused to peroxidase Orp1 resulting in an H₂O₂-sensitive probe. This application note describes how these measurements can be performed using the LVF monochromator of the CLARIOstar®.