Borealis Imaging and Illumination Upgrade for CSU Spinning Disks

Reliable and accurate quantitative comparisons of fluorescence intensities across an image are impossible when the field illumination profile is not uniform. Uneven illumination of the sample will cause the intensity of a feature in one region of the field of view to register a different magnitude than the intensity of a feature of equal fluorophore concentration in another region of the field of view. To combat this, Borealis has been developed to include a combination of optical techniques that has been shown to address the issue of uneven illumination.