Brief HPLC analysis of hydrocortisone in accordance with the United States Pharmacopeia

Hydrocortisone is the pharmaceutical term for cortisol used in oral administration, intravenous injection, or topical application. It is often used as an immunosuppressive drug, given by injection, in the treatment of severe allergic reactions, such as anaphylaxis and angioedema. The United States Pharmacopeia (USP) provides monographs for the identification and impurity testing of the drug substances. This application note describes the use of PerkinElmer Epic™ C18, Epic™ C18 SD (highly base deactivated high carbon super dense phase), and Epic^™ Polar (150 x 4.6 mm, 5 µm) columns, coupled with the PerkinElmer LC 300 liquid chromatography system and SimplicityChrom^™ Chromatography Data System (CDS), for the assay analysis of hydrocortisone.