Capability of spectral flow cytometry for resolving fluorochromes with highly overlapping spectra

3 Jul 2025

This application note highlights the Agilent NovoCyte Opteon spectral flow cytometer’s ability to resolve fluorochromes with highly overlapping spectra using carefully designed multicolor panels. Its advanced spectral unmixing capabilities enable effective separation of similar fluorochromes, allowing for greater flexibility in fluorochrome selection and expanded biomarker profiling within a single panel.

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