Capturing Native Protein States in Real-Time Using a Novel MS-Compatible Phosphatase and Protease Inhibitor Formulation

While use of stop-state inhibitor cocktails, which are designed to minimize activity from proteases and phosphatases upon sample collection, is commonplace, many formulations from commercial suppliers can lead to difficulties in fractionation of the sample or covalent modification of the sample resulting in the appearance of artifacts during mass spectrometry (MS) analyses. This poster describes a new formulation of small molecules, designed to minimize these effects, which combine phosphatase and protease inhibition in an MS-compatible format. Compatibility and effectiveness of the novel formulation is demonstrated in various workflows leading towards MS analysis.