Cationic polymer transfections in Corning HYPERFlask cell culture vessels

One of the most useful tools in cell biology research is transfection, the introduction of foreign DNA into eukaryotic cells. In this study, HeLa and CHO-K1 cells were transfected using Polyplus-transfection jetPEI^™ transfection reagent along with two different DNA constructs: one designed to test transfection via secreted protein production and the other to determine transfection efficiency through GFP expression within cells.