Cell-Based Screening of Focused Bioactive Compound Libraries: Assessing Small Molecule Modulators of the Canonical Wnt Signaling and Autophagy-Lysosome Pathways

Wnt signaling has been extensively investigated as an effector of diverse physiological processes including neuronal development and plasticity, bone development and formation, as well as pathological diseases, such as cancer and neurodegeneration. In this application note the Screen-Well® Autophagy compound library was screened using the Leading Light™ Wnt Reporter Assay Kit. Sets of Wnt agonists and antagonists were identified among the compounds. Most of the Wnt antagonists identified by this screen are known to be positive modulators of autophagy pathway or mitochondrial poisons.