Cell Cycle Analysis on Celigo

Analysis of the cell cycle is often used in drug screening to discover compounds that affect the proliferation and growth of cells. In this application note, the Expression Analysis application on the Celigo cytometer from Brooks Life Science Systems was used to monitor each phase of the cell cycle. The ability to analyze cell cycle progression of adherent cells directly in multi-well plates greatly facilitates the implementation of this assay in compound screens, significantly reduces the number of cells and reagents required for each data point, and eliminates cell physiology impacts caused by trypsinization and suspension of adherent cells.