Cell Viability Assays with Mithras LB 940

Actinomycin D is used as an anti-mitotic and an intercalating agent of DNA. It inhibits the synthesis of mRNA by intercalation into the DNA strand and thereby preventing the polymerase activity. In this application note read how the effects of two concentrations of Actinomycin D were tested on CHO cells. The cellular viability of those cells was assessed by different cell viability agents/assay kits like Alamar Blue (Invitrogen), UptiBlue (Interchim) and Resazurin (Interchim).