CellPlayer™ Kinetic Proliferation Assay

In cell proliferation assays, cells with very low contrast can prove difficult to quantify, and the relationship between confluence and true cell number becomes non-linear both when cell morphology changes and when full confluence is achieved. To resolve these issues, Essen introduced a method to measure kinetic cell proliferation based on direct cell (nuclear) counting using the IncuCyte platform. This application note demonstrates the validity and flexibility of the NucLight reagents in combination with the IncuCyte platform.