Characterization of Biotherapeutics with the ACQUITY UPLC H-Class Bio with 2D, Part 1 of 3: On-line Desalting of Biotherapeutic Samples for Increased Productivity

This application note series demonstrates that the ACQUITY UPLC H-Class Bio System with 2D technology offers advantages for the on-line fractionation and desalting of challenging biological samples and is a viable interface for IEX with MS analysis, using the therapeutic monoclonal antibody, infliximab. Characterization of charge variants in biotherapeutic proteins often relies on charge-based separation techniques such as ion exchange chromatography (IEX). While IEX separations are powerful in gathering information such as charge variant composition, the use of salts and buffer components prevents direst coupling to mass spectrometry to elucidate peak identity.