Characterization of Emission Properties of Aequorin Mutants with Rapid, High Throughput Screening Assay

This application note explains how to perform a rapid, high throughput screening assay of mutated photoprotein aequorin in order to find clones with more intense, slower kinetic, narrow spectral or color-shifted bioluminescent emission. Several examples of bioluminescent emission kinetics and spectra of mutated aequorins are included.