Characterization of hyaluronic acid with online multi-angle light scattering and differential viscometry

The biopolymer hyaluronic acid is present in all mammalian systems and is of enormous commercial interest for opthalmic, medical, pharmacological and cosmetic applications. In this application note, the absolute determination of hyaluronic acids' properties is demonstrated using MALS, differential refractometry, and differential viscometry detectors in series with SEC separation.