Characterization of immune cell phenotypes through quantification of the 12-plex spatial RNAscope HiPlex v2 assay using the HALO image analysis platform

In this application note, Advanced Cell Diagnostics demonstrates how the HALO^® image analysis platform can be employed with HiPlex v2 to quantitatively assess differential expression of 12 RNA targets and quantify six distinct cell phenotypes based on gene expression. Plus, spatial relationships between cell phenotypes within the tumor microenvironment (TME) are analyzed.