Characterizing Calcium Mobilization using Kinetic Live Cell Imaging

Monitoring calcium flux is an important method for characterizing GPCR (G-protein coupled receptor) activation. This application note describes a live cell imaging based approach to quantify Ca²⁺ flux kinetics using the Lionheart™ FX and Fluo-4 Ca²⁺ indicator dye that delivers sub-second resolution and a large assay window. This method enabled continuous monitoring of intracellular calcium, provided very good sensitivity and generated detailed kinetic profiles for GPCRs.