Charge heterogeneity characterization of an IgG4-based mAb using AEX coupled to MS

In this application note, YMC highlights that AEX-MS analysis serves as a viable alternative for characterizing charge heterogeneity in IgG4-based monoclonal antibodies (mAbs). The resolution of glycosylated variants can be enhanced through PNGase F-mediated deglycosylation. The note suggests that AEX-MS methods are well-suited for monitoring the Fc critical quality attribute of IgG4-based mAbs, whereas CEX is deemed a better option for analyzing the F(ab')2 subunit.