Chemiluminescent Western Blot Imaging using ChemiFast Substrate

Western blotting is a commonly used technique to identify and quantify specific protein(s). The commonly used western blotting substrates are luminol based and produce a chemiluminescent signal. Substrates such as ChemiFast are highly sensitive enhanced chemiluminescent substrates. This application note presents ChemiFast substrate’s extremely intense signal output, enabling detection of HRP using cooled charge couple device (CCD) camera imaging methods.