ChIP-Seq Library Prep Using the Pippin Prep DNA Size Selection System

ChIP-Seq analysis is valuable for identifying genomic DNA sequences that are associated with immunoprecipitable proteins, such as modified histones or transcription factors, in vivo. One of the technically challenging and labor-intensive steps in ChIP-Seq is the size selection of DNA fragments during the NGS library construction and is a source of sample loss and sample cross contamination. This application note compares two methods for the size-selection step.