Cloning Blunt-End Fragments of Various Sizes

Takara’s Mighty Cloning Reagent Set (Blunt End) is designed to allow PCR products to be cloned into blunt-end vectors simply and quickly. Since PCR products with or without dA termini can be blunt-ended and phosphorylated at the 5’-ends in a single reaction, DNA fragments can be ready for ligation in a simple step. Pretreatment of PCR products to inactivate enzymes or remove unused dNTPs and primers is unnecessary. The cloning efficiency of the kit was demonstrated with PCR products that were used either directly or after gel purification.