Co-culturing and Assaying Spheroids in the Corning® Spheroid Microplate

In this study, A549 cells, a human lung carcinoma cell line, were cultured in spheroid microplates with and without co-culture conditions, including primary human lung fibroblasts and peripheral blood mononuclear cells. Tumor spheroid viability was screened after treatment with various chemotherapeutics in both single culture and co-culture conditions. Dose-dependent responses of selected chemotherapeutics were compared, demonstrating the impact that including multiple cell types in 3D assays can have on therapeutic outcome.