Combining Kinetic Ligand Binding and 3D Tumor Invasion Technologies to Assess Drug Residence Time and Anti-Metastatic Effects of CXCR4 Inhibitors

The expression of chemokine receptors, such as CXCR4 and CCR7, is tightly correlated with the metastatic properties of breast cancer cells. This study demonstrates the use of the Synergy™ Neo and Cytation™ 5 Cell Imaging Multi-Mode Readers to provide PMT-based assessment of drug-target residence time of various CXCR4 inhibitors, as well as automated image-based monitoring of tumor invasion through a basement membrane matrix.