Comparing Automated vs. Manual Methods and Subsequent Recoveries of Extracted Vomitoxin from a Variety of Processed Wheat Samples

Safety testing for presence of deoxynivalenol involves a few sample preparation steps prior to analysis. Solid phase extraction is a common technique used for sample cleanup following liquid-liquid extraction of the weighed sample. Automation of this technique allows for consistent handling of samples 24/7 and without technician bias, increasing confidence in results. This application note describes the transfer of the Supelco manual deoxynivalenol SPE method to the SmartPrep Extractor automated SPE system. Five wheat samples with varying degrees of processing were tested for natural levels of deoxynivalenol, as well as spiked for determination of recovery.