Comparison of Accurate Mass and Nominal Mass MS for the Simultaneous Acquisition of Qualitative and Quantitative Data in DMPK Studies

This poster compares the benefits and drawbacks of the simultaneous acquisition of qualitative and quantitative LC/MS/MS data using either a QToF MS instrument or a triple quadrupole instrument. The throughput, sensitivity and spectral quality were also compared using a set of structurally diverse compounds prepared in rat plasma. Metabolites of one of the compounds, nefazodone, were prepared by incubation with rat liver microsomes in order to assess two different approaches to metabolite detection.