Comparison of Diagenode’s LowCell# ChIP Kit and Commercially Available Kits

Determining the genomic targets of DNA-binding proteins is crucial in understanding transcriptional control, epigenetic silencing, gene regulation pathways, and cellular proliferation. Chromatin immunoprecipitation (ChIP) allows analysis of this association of proteins with specific genomic regions in vivo. In recent years, several vendors have developed ChIP kits to overcome the tedious optimization ChIP typically requires. This study compared four commercially available ChIP kits with the Diagenode LowCell# ChIP Kit. These kits include: ChIP-IT Express Kit (Active Motif), Magna ChIP A Kit (Millipore), Imprint Chromatin Immunoprecipitation Kit (Sigma), and MAGnify Chromatin Immunoprecipitation System Kit (Invitrogen).