Comparison of ELISA, Immuno-PCR, and SIMOA immunoassay methods

Enzyme-linked immunosorbent assay (ELISA) is the gold standard method for protein detection. This resource reviews three widely used ELISA platforms: standard ELISA, immuno-PCR (IQELISA^®/BIQ-ELISA^™), and single molecule array (Simoa^®). These platforms are compared based on their design principles, sample volume requirements, instrumentation, and sensitivity.