Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer Cell Model

Epithelial-mesenchymal transition (EMT) is characterized by rearrangement of the extracellular matrix (ECM) and differential regulation of ECM proteins. This study illustrates the differences in protein expression levels and in cellular tolerance for compound treatment between a human prostate cancer cell line grown in monolayers and those same cells grown in 3D spheroids.