Comparison of limiting dilution and CellRaft technology for cell line development

In this application note, Cell Microsystems discusses the importance of therapeutic proteins in the pharmaceutical market and the need to establish high-quality monoclonal cell lines for efficient recombinant protein production. Chinese hamster ovary (CHO) cell lines are commonly used for this purpose, but generating a homogeneous cell line is challenging. Single-cell cloning is necessary, but traditional methods like limiting dilution are time-consuming and labor intensive.

CellRaft^® technology has been designed to offer a streamlined and efficient solution, combining flask-like cell culture conditions with automated isolation and imaging using the CellRaft AIR^® System. This technology improves cell line development, providing higher seeding and cloning efficiency, and supporting cell health with automated isolation for downstream propagation. The CellRaft^® Array demonstrates advantages over the traditional method, making cell line development more time, labor, and cost-efficient.