Comparison of Photina Luminescent Calcium Mobilization Assays on both the FlexStation 3 and FLIPR^TETRA Systems

This application note provides a basic protocol for performing an adherent aequorin assay using the FlexStation 3 Microplate Reader and FLIPR^TETRA System with ICCD camera. The FLIPR^TETRA® System from Molecular Devices is the market-leading instrument for monitoring GPCR and ion channel activity, and represents a reliable and flexible HTS/uHTS solution for early identification of lead compounds in the drug discovery process. The FLIPR^TETRA System is now available with an aequorin option which includes a novel ICCD camera technology optimized for use with both fluorescent and luminescent assays. Furthermore, the cell suspension system makes the system amenable to both adherent and suspension cell-based assays in 96-, 384- and 1536-well formats.