Comparison of protein detection tools

ELISA, or enzyme-linked immunosorbent assay, is the gold standard method for protein detection. In this whitepaper, RayBiotech reviews and compares three popular ELISA platforms: standard ELISA (sELISA), immuno-PCR (IQELISA™), and single molecule array (SIMOA™). The three ELISAs are compared regarding their design principles, sample volumes, instruments, and sensitivities. sELISA is the most popular method because low-cost kits are available that can target thousands of different proteins from numerous species. Moreover, it requires little training to run, its data is easy to interpret, and it uses a non-dedicated microplate reader that is compatible with other assays. However, although sELISA is the most affordable option, SIMOA is the most sensitive.