Comparison of Retrovirus-Mediated Gene Transfer into Human Hematopoietic Stem Cells using RetroNectin® Reagent vs. Polybrene

RetroNectin (Recombinant Human Fibronectin Fragment) can dramatically increase lentiviral and retroviral transduction efficiency in hard-to-infect cell types and hematopoietic stem cells. In this experiment, the efficiency of gene transfer was compared when using either a conventional polybrene method or RetroNectin reagent for retroviral transduction of CD34+ cells.