Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation

All cell-based biological experiments, from standard cell culture to primary cells for evaluating pharmacological agents, require accurate determination of cell viability. The Trypan Blue (TB) exclusion assay is one of the oldest and most common methods by which to assess cell viability. Fluorescent-based dyes now provide an alternative to this traditional method.