Comparison of two clean up techniques and a quick analysis of 39 pain management drugs from serum

In this application note, serum was cleaned up and compared with both a single protein precipitation protocol and also by the addition of a phospholipid removal product. The analysis was analyzed with a Kinetex 2.6 µm Biphenyl LC column to show the separation similarities. The phospholipid traces show the differences between the two clean up techniques and displays why Phree is a necessary addition to the clean up steps along with the comparative recoveries for the two techniques.