Competitive Analysis of a Small Molecule Model System

Surface Plasmon Resonance (SPR) is a well established technique for monitoring biomolecular interactions. However, the sensitivity of SPR is often limited when working with small molecules. In this application note, the analysis of small molecules with SPR using a competitive assay format and Episentec dyes is described. It is shown that competitive analysis with small dye labels enables both equilibrium and kinetics to be studied.